The Measurement of Lymphocyte Volume : Importance of Reference Particle Deformability and Counting Solution

We have determined the influence of reference particle deformability and suspending buffer tonicity on the measurement of lymphocyte volume by an electronic particle volume analyzer. When the volume analyzer was standardized with latex spherules having a shape factor (f.) of 1 .5. red cell volume was 96 cu m and lymphocyte volume was 289 cu m. The red cell volume corresponded closely to the true red cell volume; the true lymphocyte volume. however. was 21 8 cu sm when measured by the lymphocytocrit/lymphocyte count and 203 cu m by wet lymphocyte weight and density (mean 21 0 cu im). The difference between the electronic volume ( V.) of 289 cu Mm and true lymphocyte volume of 21 0 cu tm was due to the influence of lymphocyte deformability (shape factor) as